The study of bacteriophage began by F.w. Twort in 1915 and the lytic cycle recognized by d'Herelle in 1917. It repeated about the marine bacteriophage containg Vibrio phage by Smith and Spencers. V. furnissii and V. fluvialis are very close
phenotypically.
Gas production is the only key differential test in most clinical laboratories. V. furnissii is a new Vibro species which was formerly known as "V. fluvialis biovarⅡ", "V. fluvialis aerogenic", "V. fluvialis gas+".
The majority of the V. furnissii group isolated from patients with diarrhea and acute gastroenteritis but the role of V. furnissii was not clear. Much resaerch concerning V. furnissii and V. furnissii phage remains unknown.
Thus, author isolated two virulent phage from marine products. These 2 phages were examined the ultrastrucrure by electronmicroscopy and investigated temperature stability, pH stability, inactivation by UV irradiation, antibody production and
structural
protein analysis.
VF phages formed plaques, 2-3mm in diameter, and VF5 formed turbid piaques. VF3 had only head whereas VF5 had tail and head. Although temperature, pH, and UV sensitivity were phage specific, all phages almost inactivated above 70℃, and was
stable
in
wide pH range, pH 6.0-pH 10.0 and mostly inactivated by UV irradiation for 100 seconds.
Latent period was 60-80 minutes and burst size was 300 PFU/cell in VF3, 400PFU/cell in VF5. Serologicallly, antiserum of VF5 neutralized the VF3.
Phage DNAs were about 23kb and two all phages were digested with BamHI, BglⅢ. Structural protein analysis represented that VF phages have specific structural protein and several common mojor protein.
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